From Two Strains of Penicillium Notatum

This paper is the second of a series dealing with the analysis of penicillin by means of a chromatogram using solvent-phosphate buffer partition on a silica base. The first paper (Levi, 1948) described the development of this type of chromatogram which is based on that ofMartin & Synge (1941), and outlined the principles of its application to the study of penicillin. The work described was carried out mainly in 1943, and communicated privately to collaborating laboratories in Great Britain and the United States at the end of July in that year. Wider publication was not possible at that time owing to wartime secrecy restrictions. It deals with an examination of the crude penicillin produced by two strains of Penicillium notatum grown under different conditions, and shows clearly that the main active constituents ofthe two products are different; a fact which was not appreciated until the work was carried out. The further examination of the crude product is also described and shows the existence of still other penicillins.